GeneTex
United States (US)

SIGLEC8 antibody [C3], C-term

GTX108539_39806_IHC_19011507_410.jpg
GTX108539_39806_IFA_19011507_475.jpg
GTX108539_WB_19011507_555.jpg

Cat No. GTX108539

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

A431
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SIGLEC8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Sialic Acid Binding Ig Like Lectin 8,Saf2,Siglec-8,Siglec8L,Siglec8

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX108539_39806_IHC_19011507_410.jpg

GTX108539 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancerncer, using SIGLEC8(GTX108539) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108539_39806_IFA_19011507_475.jpg

GTX108539 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using SIGLEC8(GTX108539) antibody at 1:500 dilution.

GTX108539_WB_19011507_555.jpg

GTX108539 WB Image

Sample (30 µg of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
SIGLEC8 antibody
GTX108539 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REVIEW

SIGLEC8 antibody [C3], C-term Cat No. GTX108539

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 29-Aug-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : Human eosinophil, mononuclear cells
Amount used : 15μg
Blocking : 5% BSA, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Lane Description : Lane 1: human mononuclear cells, Lane 2- Lane 5: human eosinophils
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169