GeneTex
United States (US)

SIGLEC8 antibody, Internal

GTX88527_2257_WB_18121411_142.jpg

Cat No. GTX88527

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88527-PEP).
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-SYKSQLNYKTKQ, from the internal region of the protein sequence according to NP_055257.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Sialic Acid Binding Ig Like Lectin 8,Saf2,Siglec-8,Siglec8L,Siglec8

Background

Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).[supplied by OMIM, May 2010]

Database

Research Area

DATA IMAGES
GTX88527_2257_WB_18121411_142.jpg

GTX88527 WB Image

WB analysis of Molt-4 cell lysate using GTX88527 SIGLEC8 antibody, Internal.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329