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SIGLEC9 antibody [6D8]

Overexpression
Anti-SIGLEC9 antibody [6D8] used in Western Blot (WB). GTX83649
Anti-SIGLEC9 antibody [6D8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83649
Anti-SIGLEC9 antibody [6D8] used in Western Blot (WB). GTX83649
Anti-SIGLEC9 antibody [6D8] used in IHC (Paraffin sections) (IHC-P). GTX83649

Cat. No. GTX83649

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6D8

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Dog, Monkey
Package
100 μl ($519)
See all SIGLEC9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:50
IHC-P 1:50
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human SIGLEC9 (NP_055256) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sialic acid binding Ig like lectin 9 , CD329 , CDw329 , FOAP-9 , OBBP-LIKE , siglec-9

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

Database

Research Area

DATA IMAGES

Anti-SIGLEC9 antibody [6D8] used in Western Blot (WB). GTX83649

GTX83649 WB Image

WB analysis of various cell lines using GTX83649 SIGLEC9 antibody [6D8].
Loading : 35 ug per lane

Anti-SIGLEC9 antibody [6D8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83649

GTX83649 ICC/IF Image

ICC/IF analysis of HT29 cells using GTX83649 SIGLEC9 antibody [6D8].

Anti-SIGLEC9 antibody [6D8] used in Western Blot (WB). GTX83649

GTX83649 WB Image

Overexpression

WB analysis of HEK293T cells transfected with SIGLEC9 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83649 SIGLEC9 antibody [6D8].
Loading : 5 ug per lane

Anti-SIGLEC9 antibody [6D8] used in IHC (Paraffin sections) (IHC-P). GTX83649

GTX83649 IHC-P Image

IHC-P analysis of human breast tissue using GTX83649 SIGLEC9 antibody [6D8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519