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SIGMAR1 antibody [HL2230]

Recombinant antibody KO/KD
Anti-SIGMAR1 antibody [HL2230] used in Western Blot (WB). GTX638275
Anti-SIGMAR1 antibody [HL2230] used in IHC (Paraffin sections) (IHC-P). GTX638275
Anti-SIGMAR1 antibody [HL2230] used in Western Blot (WB). GTX638275
Anti-SIGMAR1 antibody [HL2230] used in Western Blot (WB). GTX638275
Anti-SIGMAR1 antibody [HL2230] used in Western Blot (WB). GTX638275

Cat. No. GTX638275

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2230

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SIGMAR1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human SIGMAR1.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sigma non-opioid intracellular receptor 1 , ALS16 , DSMA2 , OPRS1 , SIG-1R , SR-BP , SR-BP1 , SRBP , hSigmaR1 , sigma1R

Cellular Localization

Nucleus inner membrane , Nucleus outer membrane , Cytoplasmic vesicle , Endoplasmic reticulum membrane , Membrane , Cell junction , Cell membrane , Cell projection, growth cone , Postsynaptic density

Background

This gene encodes a receptor protein that interacts with a variety of psychotomimetic drugs, including cocaine and amphetamines. The receptor is believed to play an important role in the cellular functions of various tissues associated with the endocrine, immune, and nervous systems. As indicated by its previous name, opioid receptor sigma 1 (OPRS1), the product of this gene was erroneously thought to function as an opioid receptor; it is now thought to be a non-opioid receptor. Mutations in this gene has been associated with juvenile amyotrophic lateral sclerosis 16. Alternative splicing of this gene results in transcript variants encoding distinct isoforms. [provided by RefSeq, Aug 2013]

Database

Research Area

DATA IMAGES

Anti-SIGMAR1 antibody [HL2230] used in Western Blot (WB). GTX638275

GTX638275 WB Image

KO/KD

Wild-type (WT) and SIGMAR1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SIGMAR1 antibody [HL2230] (GTX638275) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SIGMAR1 antibody [HL2230] used in IHC (Paraffin sections) (IHC-P). GTX638275

GTX638275 IHC-P Image

SIGMAR1 antibody [HL2230] detects SIGMAR1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
SIGMAR1 stained by SIGMAR1 antibody [HL2230] (GTX638275) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SIGMAR1 antibody [HL2230] used in Western Blot (WB). GTX638275

GTX638275 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SIGMAR1 antibody [HL2230] (GTX638275) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SIGMAR1 antibody [HL2230] used in Western Blot (WB). GTX638275

GTX638275 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SIGMAR1 antibody [HL2230] (GTX638275) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SIGMAR1 antibody [HL2230] used in Western Blot (WB). GTX638275

GTX638275 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SIGMAR1 antibody [HL2230] (GTX638275) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations