Wild-type (WT) and SIGMAR1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SIGMAR1 antibody [HL2230] (GTX638275) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SIGMAR1 antibody [HL2230] (GTX638275) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SIGMAR1 antibody [HL2230] detects SIGMAR1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
SIGMAR1 stained by SIGMAR1 antibody [HL2230] (GTX638275) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SIGMAR1 antibody [HL2230] (GTX638275) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SIGMAR1 antibody [HL2230] (GTX638275) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.