GeneTex
United States (US)

SIGMAR1 antibody [N1C3]

KO/KD
GTX115389_40302_20180216_WB_KO_watermark_18121410_624.jpg
GTX115389_40302_IHC_18121410_265.jpg

Cat No. GTX115389

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Zebrafish
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Rat, Bovine, Guinea pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SIGMAR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Sigma Non-Opioid Intracellular Receptor 1,Als16,Dsma2,Oprs1,Sig-1R,Sr-Bp,Sr-Bp1,Srbp,Hsigmar1,Sigma1R,Sigmar1

Cellular Localization

Nucleus inner membrane , Nucleus outer membrane , Endoplasmic reticulum membrane , Lipid droplet , Cell junction , Cell membrane , Cell projection , growth cone

Background

This gene encodes a receptor protein that interacts with a variety of psychotomimetic drugs, including cocaine and amphetamines. The receptor is believed to play an important role in the cellular functions of various tissues associated with the endocrine, immune, and nervous systems. As indicated by its previous name, opioid receptor sigma 1 (OPRS1), the product of this gene was erroneously thought to function as an opioid receptor; it is now thought to be a non-opioid receptor. Alternative splicing of this gene results in transcript variants encoding distinct isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX115389_40302_20180216_WB_KO_watermark_18121410_624.jpg

GTX115389 WB Image

KO/KD

Wild-type (WT) and SIGMAR1 knockout (KO) 293T cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with SIGMAR1 antibody [N1C3] (GTX115389) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX115389_40302_IHC_18121410_265.jpg

GTX115389 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using SIGMAR1(GTX115389) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169