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SIL1 antibody [N1C2]

Anti-SIL1 antibody [N1C2] used in Western Blot (WB). GTX116756
Anti-SIL1 antibody [N1C2] used in Western Blot (WB). GTX116756
Anti-SIL1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX116756

Cat. No. GTX116756

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all SIL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

A431 , rat liver

Predict Reactivity

Mouse, Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.92 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SIL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10615363

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SIL1 nucleotide exchange factor , BAP , MSS , ULG5

Cellular Localization

Endoplasmic reticulum lumen

Background

This gene encodes a resident endoplasmic reticulum (ER), N-linked glycoprotein with an N-terminal ER targeting sequence, 2 putative N-glycosylation sites, and a C-terminal ER retention signal. This protein functions as a nucleotide exchange factor for another unfolded protein response protein. Mutations in this gene have been associated with Marinesco-Sjogren syndrome. Alternate transcriptional splice variants have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SIL1 antibody [N1C2] used in Western Blot (WB). GTX116756

GTX116756 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX116756 diluted at 1:5000

Anti-SIL1 antibody [N1C2] used in Western Blot (WB). GTX116756

GTX116756 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SIL1 antibody [N1C2] (GTX116756) diluted at 1:5000.

Anti-SIL1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX116756

GTX116756 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SIL1(GTX116756) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Cunningham CN et al. Diabetes 2016; (Epub) chaperone-driven degradation of a misfolded pro* mutant in parallel with restoration of wild type * secretion.
Application : WB
Reactivity : Human
Inoue T et al. Mol Biol Cell 2016; (Epub) The Grp170 nucleotide exchange factor executes a key role during ERAD of cellular misfolded clients.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169