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SIL1 antibody [N1N3]

Anti-SIL1 antibody [N1N3] used in Western Blot (WB). GTX116755
Anti-SIL1 antibody [N1N3] used in Western Blot (WB). GTX116755
Anti-SIL1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX116755
Anti-SIL1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX116755

Cat. No. GTX116755

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all SIL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

A431 , rat kidney

Predict Reactivity

Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SIL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617803

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SIL1 nucleotide exchange factor , BAP , MSS , ULG5

Background

This gene encodes a resident endoplasmic reticulum (ER), N-linked glycoprotein with an N-terminal ER targeting sequence, 2 putative N-glycosylation sites, and a C-terminal ER retention signal. This protein functions as a nucleotide exchange factor for another unfolded protein response protein. Mutations in this gene have been associated with Marinesco-Sjogren syndrome. Alternate transcriptional splice variants have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SIL1 antibody [N1N3] used in Western Blot (WB). GTX116755

GTX116755 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX116755 diluted at 1:5000

Anti-SIL1 antibody [N1N3] used in Western Blot (WB). GTX116755

GTX116755 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SIL1 antibody [N1N3] (GTX116755) diluted at 1:5000.

Anti-SIL1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX116755

GTX116755 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using SIL1(GTX116755) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SIL1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX116755

GTX116755 IHC-P Image

SIL1 antibody [N1N3] detects SIL1 protein at cytoplasm in rat muscle by immunohistochemical analysis.
Sample: Paraffin-embedded rat muscle.
SIL1 antibody [N1N3] (GTX116755) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Inoue T et al. J Virol 2015; 89 (8) : 4069-79 A nucleotide exchange factor promotes endoplasmic reticulum-to-cytosol membrane penetration of the nonenveloped virus simian virus 40.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169