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SIN3A antibody

KO/KD Orthogonal
Anti-SIN3A antibody used in Western Blot (WB). GTX129156
Anti-SIN3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129156
Anti-SIN3A antibody used in IHC (Paraffin sections) (IHC-P). GTX129156
Anti-SIN3A antibody used in Western Blot (WB). GTX129156
Anti-SIN3A antibody used in IHC (Paraffin sections) (IHC-P). GTX129156
Anti-SIN3A antibody used in Immunoprecipitation (IP). GTX129156
Anti-SIN3A antibody used in IHC (Paraffin sections) (IHC-P). GTX129156
Anti-SIN3A antibody used in Western Blot (WB). GTX129156
Anti-SIN3A antibody used in ChIP assay (ChIP assay). GTX129156

Cat. No. GTX129156

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SIN3A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

145 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse brain , rat brain

Product Note

Knockdown/Knockout validation was supported by references (PMID:28805661)

Predict Reactivity

Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SIN3A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885913

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SIN3 transcription regulator family member A , WITKOS

Cellular Localization

Nucleus , nucleolus

Background

The protein encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SIN3A antibody used in Western Blot (WB). GTX129156

GTX129156 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SIN3A antibody (GTX129156) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SIN3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129156

GTX129156 ICC/IF Image

SIN3A antibody detects SIN3A protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SIN3A stained by SIN3A antibody (GTX129156) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.

Anti-SIN3A antibody used in IHC (Paraffin sections) (IHC-P). GTX129156

GTX129156 IHC-P Image

SIN3A antibody detects SIN3A protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
SIN3A antibody (GTX129156) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SIN3A antibody used in Western Blot (WB). GTX129156

GTX129156 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SIN3A antibody (GTX129156) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SIN3A antibody used in IHC (Paraffin sections) (IHC-P). GTX129156

GTX129156 IHC-P Image

SIN3A antibody detects SIN3A protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
SIN3A antibody (GTX129156) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SIN3A antibody used in Immunoprecipitation (IP). GTX129156

GTX129156 IP Image

SIN3A antibody immunoprecipitates SIN3A protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of SIN3A protein expressing 293T cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of SIN3A by 3 μg of SIN3A antibody (GTX129156) 5% SDS-PAGE The immunoprecipitated SIN3A protein was detected by SIN3A antibody (GTX129156) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-SIN3A antibody used in IHC (Paraffin sections) (IHC-P). GTX129156

GTX129156 IHC-P Image

SIN3A antibody detects SIN3A protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
SIN3A antibody (GTX129156) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SIN3A antibody used in Western Blot (WB). GTX129156

GTX129156 WB Image

SIN3A antibody detects SIN3A protein by western blot analysis.
A 293T whole cell lysate/extract
B A431 whole cell lysate/extract
C HeLa whole cell lysate/extract
D HepG2 whole cell lysate/extract
5% SDS-PAGE
SIN3A antibody (GTX129156) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SIN3A antibody used in ChIP assay (ChIP assay). GTX129156

GTX129156 ChIP assay Image

Cross-linked ChIP was performed with MCF-7 chromatin extract treated with B-estradiol (10 nM for 45 min) and 5 μg of either control rabbit IgG or anti-SIN3A antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or MyoD.

REFERENCE

Application Reference

Chao Yang et al. bioRxiv 2020; (Epub) Circadian Rhythm Is Disrupted by ZNF704 in Breast Carcinogenesis.
Application : WB
Reactivity : Human
Li W et al. J Clin Invest 2017; (Epub) The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169