IHC-P analysis of human ovary carcinoma tissue using GTX23479 SIN3A antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200

ICC/IF analysis of NIH-3T3 cells using GTX23479 SIN3A antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 and incubated overnight at 4ºC

WB analysis of K562 cell extract using GTX23479 SIN3A antibody.

ICC/IF analysis of A431 cells using GTX23479 SIN3A antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 and incubated overnight at 4ºC

ICC/IF analysis of Hela cells using GTX23479 SIN3A antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4ºC

ChIP analysis of 1x10⁶ HCT116 colon carcinoma cells treated with serum for 0, 15, and 30 minutes using GTX23479 SIN3A antibody. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (refer to PMID: 22098709). The precipitated DNA was detected by PCR with primer set targeting to -15kb upstream of the Egr1 gene or exon-1 or exon-2 of Egr1.
IP reaction : 1.0ul antibody / 100ul sample