GeneTex

  • Country / Location Selection

United States (US)

SIN3A antibody

Orthogonal
Anti-SIN3A antibody used in ChIP assay (ChIP assay). GTX23479
Anti-SIN3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23479
Anti-SIN3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23479
Anti-SIN3A antibody used in Western Blot (WB). GTX23479
Anti-SIN3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23479
Anti-SIN3A antibody used in IHC (Paraffin sections) (IHC-P). GTX23479

Cat. No. GTX23479

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all SIN3A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 μg/ml
ICC/IF 1:50-1:500
IHC-P 1:50-1:500
IP Assay dependent
ChIP assay 1-3 μl
Not tested in other applications.

Calculated MW

145 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to residues M(1) K R R L D D Q E S D V Y A A Q Q R R(19) of mouse mSin3A.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SIN3 transcription regulator family member A , WITKOS

Cellular Localization

Nucleus,Nucleolus

Background

The protein encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SIN3A antibody used in ChIP assay (ChIP assay). GTX23479

GTX23479 ChIP assay Image

Orthogonal

ChIP analysis of 1x10⁶ HCT116 colon carcinoma cells treated with serum for 0, 15, and 30 minutes using GTX23479 SIN3A antibody. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (refer to PMID: 22098709). The precipitated DNA was detected by PCR with primer set targeting to -15kb upstream of the Egr1 gene or exon-1 or exon-2 of Egr1.
IP reaction : 1.0ul antibody / 100ul sample

Anti-SIN3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23479

GTX23479 ICC/IF Image

ICC/IF analysis of Hela cells using GTX23479 SIN3A antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4ºC

Anti-SIN3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23479

GTX23479 ICC/IF Image

ICC/IF analysis of A431 cells using GTX23479 SIN3A antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 and incubated overnight at 4ºC

Anti-SIN3A antibody used in Western Blot (WB). GTX23479

GTX23479 WB Image

WB analysis of K562 cell extract using GTX23479 SIN3A antibody.

Anti-SIN3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23479

GTX23479 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX23479 SIN3A antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 and incubated overnight at 4ºC

Anti-SIN3A antibody used in IHC (Paraffin sections) (IHC-P). GTX23479

GTX23479 IHC-P Image

IHC-P analysis of human ovary carcinoma tissue using GTX23479 SIN3A antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200

REFERENCE

There are currently no references for SIN3A antibody (GTX23479). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for SIN3A antibody (GTX23479). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399