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SIN3B antibody [N1N2-2], N-term

Anti-SIN3B antibody [N1N2-2], N-term used in Western Blot (WB). GTX116417
Anti-SIN3B antibody [N1N2-2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116417
Anti-SIN3B antibody [N1N2-2], N-term used in Western Blot (WB). GTX116417
Anti-SIN3B antibody [N1N2-2], N-term used in Immunoprecipitation (IP). GTX116417

Cat. No. GTX116417

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SIN3B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

133 kDa. ( Note )

Positive Control

Mouse brain , HCT116

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SIN3B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10616827

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SIN3 transcription regulator family member B

Cellular Localization

Nucleus

Background

Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription.

Database

Research Area

DATA IMAGES

Anti-SIN3B antibody [N1N2-2], N-term used in Western Blot (WB). GTX116417

GTX116417 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
10% SDS PAGE
GTX116417 diluted at 1:1000

Anti-SIN3B antibody [N1N2-2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116417

GTX116417 ICC/IF Image

SIN3B antibody [N1N2-2], N-term detects SIN3B protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SIN3B protein stained by SIN3B antibody [N1N2-2], N-term (GTX116417) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-SIN3B antibody [N1N2-2], N-term used in Western Blot (WB). GTX116417

GTX116417 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
5% SDS PAGE
GTX116417 diluted at 1:500

Anti-SIN3B antibody [N1N2-2], N-term used in Immunoprecipitation (IP). GTX116417

GTX116417 IP Image

Immunoprecipitation of SIN3B protein from HCT-116 whole cell extracts using 5 μg of SIN3B antibody [N1N2-2], N-term (GTX116417).
Western blot analysis was performed using SIN3B antibody [N1N2-2], N-term (GTX116417) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169