SIN3B antibody [N1N2], N-term

Cat. No. GTX116326

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all SIN3B products

Datasheet
Document

View product citations for antibody GTX116326 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

133 kDa. ( Note )

Positive Control

HCT116

Predict Reactivity

Mouse, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.96 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SIN3B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617287

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SIN3 transcription regulator family member B

Cellular Localization

Nucleus

Background

Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription.

Database

Gene ID: 23309 SIN3B
UniProt: O75182 SIN3B

Research Area

DATA IMAGES

GTX116326 IP Image

Immunoprecipitation of SIN3B protein from HCT-116 whole cell extracts using 5 μg of SIN3B antibody [N1N2], N-term (GTX116326).
Western blot analysis was performed using SIN3B antibody [N1N2], N-term (GTX116326) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX116326 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using SIN3B(GTX116326) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX116326 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
5% SDS PAGE
GTX116326 diluted at 1:500

GTX116326 ICC/IF Image

SIN3B antibody [N1N2], N-term detects SIN3B protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SIN3B protein stained by SIN3B antibody [N1N2], N-term (GTX116326) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty