Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SIN3B antibody [N1N2], N-term (GTX116326) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

SIN3B antibody [N1N2], N-term detects SIN3B protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SIN3B protein stained by SIN3B antibody [N1N2], N-term (GTX116326) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using SIN3B(GTX116326) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunoprecipitation of SIN3B protein from HCT-116 whole cell extracts using 5 μg of SIN3B antibody [N1N2], N-term (GTX116326).
Western blot analysis was performed using SIN3B antibody [N1N2], N-term (GTX116326) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.