GeneTex
United States (US)

SIPA1 antibody, C-term

GTX10162_1461_WB_19011506_541.jpg

Cat No. GTX10162

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
Not tested in other applications.

Calculated MW

112 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX10162-PEP).
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-ASKQLGSPTADLA, from the C Terminus of the protein sequence according to NP_694985.28; NP_006738.3.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Signal-Induced Proliferation-Associated 1,Spa1,Sipa1

Cellular Localization

Perinuclear

Background

The product of this gene is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX10162_1461_WB_19011506_541.jpg

GTX10162 WB Image

WB analysis of human EBV-immortilized lymphoblastoid lysatewith (B) and without (A) blocking with the immunising peptide using GTX10162 SIPA1 antibody, C-term.
Dilution : 1µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329