GeneTex
United States (US)

SIPA1 antibody [C3], C-term

GTX104786_39680_IFA_mESC_19011506_769.jpg
GTX104786_WB_19011506_965.jpg
GTX104786_39680_IHC_19011506_443.jpg
GTX104786_39680_IHC_2_19011506_801.jpg

Cat No. GTX104786

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

112 kDa. ( Note )

Positive Control

SIPA1 tansfected 293T cell

Predict Reactivity

Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SIPA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC102688 antibody, MGC17037 antibody, SPA1 antibody, SIPA1 antibody, p130 SPA-1 antibody, sipa-1 antibody, GTPase-activating protein Spa-1 antibody, signal-induced proliferation-associated protein 1 antibody, signal-induced proliferation-associated gene 1 antibody, signal-induced proliferation-associated 1 antibody

Cellular Localization

Nucleus , Cytoplasm , perinuclear region , Endomembrane system; Peripheral membrane protein

Background

The product of this gene is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date. [provided by RefSeq]
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Database

DATA IMAGES
GTX104786_39680_IFA_mESC_19011506_769.jpg

GTX104786 ICC/IF Image

Immunofluorescence analysis of methanol-fixed mouse ESC, using SIPA1(GTX104786) antibody at 1:200 dilution.

GTX104786_WB_19011506_965.jpg

GTX104786 WB Image

Western Blot analysis of SIPA1 expression in transfected 293T cell line by SIPA1 polyclonal antibody.
A: Non-transfected lysate.
B: SIPA1 transfected lysate.
7.5% SDS PAGE
GTX104786 diluted at 1:500

GTX104786_39680_IHC_19011506_443.jpg

GTX104786 IHC-P Image

SIPA1 antibody [C3], C-term detects SIPA1 protein at weak membrane and cytosol on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
SIPA1 antibody [C3], C-term (GTX104786) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104786_39680_IHC_2_19011506_801.jpg

GTX104786 IHC-P Image

SIPA1 antibody [C3], C-term detects SIPA1 protein at weak membrane and cytosol on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
SIPA1 antibody [C3], C-term (GTX104786) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169