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SIPA1 antibody [C3], C-term

Anti-SIPA1 antibody [C3], C-term used in Western Blot (WB). GTX104786
Anti-SIPA1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104786
Anti-SIPA1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104786
Anti-SIPA1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104786

Cat. No. GTX104786

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SIPA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

112 kDa. ( Note )

Positive Control

SIPA1 transfected 293T

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SIPA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241331

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

signal-induced proliferation-associated 1 , SPA1

Cellular Localization

Nucleus , Cytoplasm , perinuclear region , Endomembrane system; Peripheral membrane protein

Background

The product of this gene is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SIPA1 antibody [C3], C-term used in Western Blot (WB). GTX104786

GTX104786 WB Image

Western Blot analysis of SIPA1 expression in transfected 293T cell line by SIPA1 polyclonal antibody.
A: Non-transfected lysate.
B: SIPA1 transfected lysate.
7.5% SDS PAGE
GTX104786 diluted at 1:500

Anti-SIPA1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104786

GTX104786 IHC-P Image

SIPA1 antibody [C3], C-term detects SIPA1 protein at weak membrane and cytosol on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
SIPA1 antibody [C3], C-term (GTX104786) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SIPA1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104786

GTX104786 ICC/IF Image

Immunofluorescence analysis of methanol-fixed mouse ESC, using SIPA1(GTX104786) antibody at 1:200 dilution.

Anti-SIPA1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104786

GTX104786 IHC-P Image

SIPA1 antibody [C3], C-term detects SIPA1 protein at weak membrane and cytosol on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
SIPA1 antibody [C3], C-term (GTX104786) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169