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SIRP alpha antibody

Orthogonal
Anti-SIRP alpha antibody used in Western Blot (WB). GTX112645
Anti-SIRP alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112645
Anti-SIRP alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX112645
Anti-SIRP alpha antibody used in Western Blot (WB). GTX112645
Anti-SIRP alpha antibody used in Western Blot (WB). GTX112645
Anti-SIRP alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX112645
Anti-SIRP alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX112645

Cat. No. GTX112645

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SIRP alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human SIRP alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166328

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

signal regulatory protein alpha , BIT , CD172A , MFR , MYD-1 , P84 , PTPNS1 , SHPS1 , SIRP

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SIRP alpha antibody used in Western Blot (WB). GTX112645

GTX112645 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SIRP alpha antibody (GTX112645) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SIRP alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112645

GTX112645 ICC/IF Image

SIRP alpha antibody detects SIRP alpha protein at cell membrane by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SIRP alpha stained by SIRP alpha antibody (GTX112645) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (GTX102079) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SIRP alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX112645

GTX112645 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SIRP alpha(GTX112645) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SIRP alpha antibody used in Western Blot (WB). GTX112645

GTX112645 WB Image

Orthogonal

The data was published in the journal J Neuroinflammation in 2016.PMID: 27095555

Anti-SIRP alpha antibody used in Western Blot (WB). GTX112645

GTX112645 WB Image

Orthogonal

The data was published in the journal J Neurooncol in 2020. PMID: 32020472

Anti-SIRP alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX112645

GTX112645 IHC-P Image

The data was published in the journal J Neuroinflammation in 2016.PMID: 27095555

Anti-SIRP alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX112645

GTX112645 IHC-P Image

The data was published in the journal J Neuroinflammation in 2016.PMID: 27095555

REFERENCE

Application Reference

Hsu SPC et al. J Neurooncol 2020; 146 (3) : 417-426 Rapamycin and hydroxychloroquine combination alters macrophage polarization and sensitizes glioblastoma to immune checkpoint inhibitors.
Application : WB
Reactivity : Mouse
Cabrales P et al. Transl Oncol 2019; 12 (4) : 626-632 RRx-001 Acts as a Dual Small Molecule Checkpoint Inhibitor by Downregulating CD47 on Cancer Cells and SIRP-α on Monocytes/Macrophages.
Application : WB
Reactivity : Mouse
Sun FJ et al. J Neuroinflammation 2016; 13 (1) : 85 Downregulation of CD47 and CD200 in patients with focal cortical dysplasia type IIb and tuberous sclerosis complex.
Application : WB, IHC-P
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169
Currently in production