GeneTex
United States (US)

SIRP alpha antibody

Cat No. GTX28120

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

Western blot at1:1000 to 1:2000 dilution, and 75-110 kDa bandsshould be detected.

Calculated MW

55 kDa. ( Note )

Positive Control

THP-1 whole cell lysate

Specificity/Sensitivity

Recognizes SIRPa1, 2 and 3.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide corresponding to amino acids 487 to 503 of human SIRPa1 (1). The sequences of immunogenicpeptide differ from those of mouse, rat and bovine by oneamino acid (1-7).

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Signal Regulatory Protein Alpha,Bit,Cd172A,Mfr,Myd-1,P84,Ptpns1,Shps1,Sirp,Sirpa

Cellular Localization

Type 1 membrane protein

Background

Protein tyrosine phosphatases (PTPases) SHP-1 andSHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPa (1), SHPS-1 (2,3), MyD-1 (4), BIT (5,6) and p84 (7). SIRPa is a new gene family containing at least fifteen members. SIRPa is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2 (1,2,5,8). SIRPa has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes (1,2,5,8).

Database

Research Area

Package List Price ($)
$ 389