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SIRP alpha antibody

Anti-SIRP alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64435
Anti-SIRP alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64435
Anti-SIRP alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64435
Anti-SIRP alpha antibody used in Western Blot (WB). GTX64435

Cat. No. GTX64435

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all SIRP alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 220-375 of human SIRPA (NP_542970.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

signal regulatory protein alpha , BIT , CD172A , MFR , MYD-1 , P84 , PTPNS1 , SHPS1 , SIRP

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SIRP alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64435

GTX64435 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX64435 SIRP alpha antibody.
Blue : DAPI
Dilution : 1:100

Anti-SIRP alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64435

GTX64435 ICC/IF Image

ICC/IF analysis of L929 cells using GTX64435 SIRP alpha antibody.
Blue : DAPI
Dilution : 1:100

Anti-SIRP alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64435

GTX64435 ICC/IF Image

ICC/IF analysis of H9C2 cells using GTX64435 SIRP alpha antibody.
Blue : DAPI
Dilution : 1:100

Anti-SIRP alpha antibody used in Western Blot (WB). GTX64435

GTX64435 WB Image

WB analysis of various sample lysates using GTX64435 SIRP alpha antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399