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SIRT1 antibody [1F3]

Anti-SIRT1 antibody [1F3] used in IHC (Paraffin sections) (IHC-P). GTX82793
Anti-SIRT1 antibody [1F3] used in Flow cytometry (FACS). GTX82793
Anti-SIRT1 antibody [1F3] used in Western Blot (WB). GTX82793
Anti-SIRT1 antibody [1F3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82793

Cat. No. GTX82793

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1F3

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Rat
Package
100 μl ($419)
See all SIRT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human SIRT1 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_11170045

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sirtuin 1 , SIR2 , SIR2L1 , SIR2alpha

Cellular Localization

Cytoplasm,Nucleus,Mitochondrion

Background

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES

Anti-SIRT1 antibody [1F3] used in IHC (Paraffin sections) (IHC-P). GTX82793

GTX82793 IHC-P Image

IHC-P analysis of lung cancer tissue (left) and kidney cancer tissue (right) using GTX82793 SIRT1 antibody [1F3].

Anti-SIRT1 antibody [1F3] used in Flow cytometry (FACS). GTX82793

GTX82793 FACS Image

FACS analysis of K562 cells using GTX82793 SIRT1 antibody [1F3].
Green : SIRT1
Purple : negative control

Anti-SIRT1 antibody [1F3] used in Western Blot (WB). GTX82793

GTX82793 WB Image

WB analysis of MCF-7 (1), Jurkat (2), HeLa (3), HEK293 (4) and A549 (5) cell lysate using GTX82793 SIRT1 antibody [1F3].

Anti-SIRT1 antibody [1F3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82793

GTX82793 ICC/IF Image

ICC/IF analysis of NTERA-2 cells using GTX82793 SIRT1 antibody [1F3].
Green : SIRT1
Red: Actin filaments

REFERENCE

Application Reference

Wan-Ping Su et al. Biomed Pharmacother 2023; 163 Boosting mitochondrial function and metabolism in aging female germ cells with dual ROCK/ROS inhibition
Application : WB
Reactivity : Human
Miler M et al. J Agric Food Chem 2020; 68 (31) : 8242-8254 Citrus Flavanones Upregulate Thyrotroph Sirt1 and Differently Affect Thyroid Nrf2 Expressions in Old-Aged Wistar Rats.
Application : IHC-P
Reactivity : Rat
Kornelius E et al. CNS Neurosci Ther 2015; 21 (7) : 549-57 DPP-4 Inhibitor Linagliptin Attenuates Aβ-induced Cytotoxicity through Activation of AMPK in Neuronal Cells.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 419