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SIRT1 antibody, N-term

Anti-SIRT1 antibody, N-term used in Western Blot (WB). GTX30870
Anti-SIRT1 antibody, N-term used in Western Blot (WB). GTX30870

Cat No. GTX30870

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μg ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2-2.5 ug/ml
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Positive Control

786-O , human fetal thymus

PROPERTIES

Form

Liquid

Buffer

1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a N-terminal region of Human SIRT1.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

sirtuin 1 , SIR2 , SIR2L1 , SIR2alpha

Cellular Localization

Cytoplasm,Nucleus,Mitochondrion

Background

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES

Anti-SIRT1 antibody, N-term used in Western Blot (WB). GTX30870

GTX30870 WB Image

WB analysis of human fetal thymus tissue using GTX30870 Sirt1 antibody at 0.5μg/ml.

Anti-SIRT1 antibody, N-term used in Western Blot (WB). GTX30870

GTX30870 WB Image

WB analysis of 786-O cells using GTX30870 Sirt1 antibody at 1μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf