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SIRT1 antibody

KO/KD Orthogonal
Anti-SIRT1 antibody used in Western Blot (WB). GTX134606
Anti-SIRT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX134606
Anti-SIRT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134606
Anti-SIRT1 antibody used in Western Blot (WB). GTX134606

Cat. No. GTX134606

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SIRT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SIRT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887317

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sirtuin 1 , SIR2 , SIR2L1 , SIR2alpha

Cellular Localization

Cytoplasm,Nucleus,Mitochondrion

Background

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES

Anti-SIRT1 antibody used in Western Blot (WB). GTX134606

GTX134606 WB Image

KO/KD

Wild-type (WT) and SIRT1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SIRT1 antibody (GTX134606) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SIRT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX134606

GTX134606 IHC-P Image

SIRT1 antibody detects SIRT1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
SIRT1 stained by SIRT1 antibody (GTX134606) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SIRT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134606

GTX134606 ICC/IF Image

SIRT1 antibody detects SIRT1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SIRT1 stained by SIRT1 antibody (GTX134606) diluted at 1:500.

Anti-SIRT1 antibody used in Western Blot (WB). GTX134606

GTX134606 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SIRT1 antibody (GTX134606) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

Application Reference

Chien LH et al. Antioxidants (Basel) 2021; 10 (10)
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

SIRT1 antibody Cat. No. GTX134606

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-SIRT1 antibody used in Western Blot (WB). GTX134606
Date : Anonymous submitted on 10-Feb-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549/ABCA3 cells
Amount used : 15μg
Blocking : 0.1% skim milk, 24°C, 1Hr
Primary Antibody : 1:1000 in Can Get Signal, 2°C, 24Hr
Submit a Review
Application Reference
Chien LH et al. Antioxidants (Basel) 2021; 10 (10)
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169