GeneTex

  • Country / Location Selection

United States (US)

SIRT1 antibody

KO/KD
Anti-SIRT1 antibody used in Immunoprecipitation (IP). GTX17532
Anti-SIRT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17532
Anti-SIRT1 antibody used in Western Blot (WB). GTX17532
Anti-SIRT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX17532
Anti-SIRT1 antibody used in Western Blot (WB). GTX17532

Cat. No. GTX17532

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all SIRT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 448-747 of human SIRT1 (NP_036370.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887566

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sirtuin 1 , SIR2 , SIR2L1 , SIR2alpha

Cellular Localization

Cytoplasm,Nucleus,Mitochondrion

Background

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES

Anti-SIRT1 antibody used in Immunoprecipitation (IP). GTX17532

GTX17532 IP Image

IP analysis of Jurkat cell lysate using GTX17532 SIRT1 antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-SIRT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17532

GTX17532 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX17532 SIRT1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-SIRT1 antibody used in Western Blot (WB). GTX17532

GTX17532 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX17532 SIRT1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-SIRT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX17532

GTX17532 IHC-P Image

IHC-P analysis of human colon tissue using GTX17532 SIRT1 antibody.
Dilution : 1:200

Anti-SIRT1 antibody used in Western Blot (WB). GTX17532

GTX17532 WB Image

WB analysis of various sample lysates using GTX17532 SIRT1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

Application Reference

Huang C et al. Pharmaceuticals (Basel) 2023; 16 (5) α-Viniferin-Induced Apoptosis through Downregulation of SIRT1 in Non-Small Cell Lung Cancer Cells.
Laura Braud et al. Redox Biol 2020; 38 : 101805 Increased Sirt1 secreted from visceral white adipose tissue is associated with improved glucose tolerance in obese Nrf2-deficient mice.
Application : IHC-P
Reactivity : Mouse
Ruiz Ram穩rez A et al. Am J Physiol Renal Physiol 2019; (Epub) Kidney dysfunction induced by sucrose-rich diet in rat involves mitochondria ROS generation, cardiolipin changes and the decline of autophagy protein markers.
Application : WB
Reactivity : Rat
Kornelius E et al. J Cell Mol Med 2018; (Epub) Liraglutide protects against glucolipotoxicity-induced RIN-m5F β-cell apoptosis through restoration of PDX1 expression.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

There are currently no reviews for SIRT1 antibody (GTX17532). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399