APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1 μg/mL |
20 μg/mL |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Mouse Liver Tissue Lysate(GTX27935)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
SIRT1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human SIRT1.The immunogen is located within the first 50 amino acids of SIRT1.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
sirtuin 1 , SIR2 , SIR2L1 , SIR2alpha
Cellular Localization
Cytoplasm,Nucleus,Mitochondrion
Background
The Silent Information Regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and have important functions in the regulation of metabolism, growth and differentiation, inflammation, cellular survival, as well as in senescence and lifespan extension. Sirtuins, including SIRT1-7, are human homologs of yeast Sir2p. Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) which regulate cellular metabolism, e.g. energy metabolism, and thereby are associated with aging and several age-related diseases. SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically via lysine residue.
Database
Research Area
DATA IMAGES
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GTX85106 WB Image
WB analysis of mouse liver tissue lysate using GTX85106 SIRT1 antibody. Working concentration : 1 μg/ml
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GTX85106 IHC-P Image
IHC-P analysis of rat lung tissue using GTX85106 SIRT1 antibody. Working concentration : 20 μg/ml
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REFERENCE
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REVIEW
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