APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:2,000 |
1:50 |
Not tested in other applications.
Calculated MW
Positive Control
NIH3T3
Product Note
Anti-Sir2 polyclonal antibody specifically recognizes mouse Sir2 (AS-16). Staining of the Sir2 band is specifically inhibited by the immunizing peptide.
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
synthetic peptide corresponding to mouse Sir2 sequence (amino acids 722-737 with N-terminal added lysine), conjugated to KLH. This sequence is 62% homologous to the corresponding human sequence.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
sirtuin 2 , 5730427M03Rik , SIR2L2 , Sir2l
Cellular Localization
Nucleus,Cytoplasm,Cytoplasm, cytoskeleton
Background
SIR2, one of the silent information regulator genes, encodes a protein that promotes a compact chromatin structure, thereby preventing or silencing gene transcription at selected loci. SIR2 belongs to a family of proteins that is found in organisms ranging from bacteria to complex eukaryotes. Members of this family contain a 250 amino acid core domain that shares about 25-60% sequence identity. Silencing occurs as a series of events initiated by formation of Sir complexes (Sir2, Sir3, Sir4). The complexes are recruited to their chromosome targets via interactions with DNA-binding proteins, followed by deacetylation of histones H3 and H4. A final step required for telomeric silencing is binding of the complex to the deacetylated histones and recruitment of the telosome to the nuclear periphery. Sir2 protein is an NAD-dependent histone deacetylase, an enzyme that removes acetyl groups from lysine residues of histone proteins and possibly other substrates. Sir2 transfers acetyl groups from its protein substrates to ADP-ribose and synthesizes o-acetyl-ADP-ribose. Through histone deacetylation, Sir2 may silence chromatin. The maintenance or silencing of chromatin may be at the center of processes leading to aging of cells and development of cancer.
Database
Research Area
DATA IMAGES
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GTX12193 ICC/IF Image
ICC/IF analysis of NIH3T3 cells using GTX12193 SIRT2 antibody at 20 μg/mL(green) with DAPI(blue).
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GTX12193 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX12193 SIRT2 antibody at 20 μg/mL(green) with DAPI(blue).
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GTX12193 WB Image
WB analysis of (lanes 1-2) HeLa nuclear extract (lanes 3-4) of HCT-116 cell lysate using GTX12193 SIRT2 antibody.
Lane 1, 3 : Antibody 1 μg/mL
Lane 2, 4 : Antibody 1 μg/mL + Sir2 immunizing peptide
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GTX12193 WB Image
WB analysis of NIH3T3 cell lysate using GTX12193 SIRT2 antibody. Lane 1 : Antibody 0.25 μg/mL
Lane 2 : Antibody 0.5 μg/mL
Lane 3 : Negative control: without first antibody
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REFERENCE
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REVIEW
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