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SIRT2 antibody

Anti-SIRT2 antibody used in Western Blot (WB). GTX129154

Cat. No. GTX129154

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

PC12

Predict Reactivity

Human, Mouse, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SIRT2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885912

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sirtuin 2 , SIR2 , SIR2L , SIR2L2

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two transcript variants result from alternative splicing of this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SIRT2 antibody used in Western Blot (WB). GTX129154

GTX129154 WB Image

SIR2 antibody detects SIR2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SIR2 antibody (GTX129154) diluted by 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169