GeneTex
United States (US)

SIRT3 antibody [C3], C-term

GTX106292_IFA_19011506_996.jpg
GTX106292_39701_IHC_19011506_323.jpg
GTX106292_WB_19011506_945.jpg
GTX106292_42172_20151013_IHC-P_19011506_670.jpg

Cat No. GTX106292

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

HepG2
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SIRT3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

SIR2L3 antibody, SIRT3 antibody, sir2-like 3 antibody, mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase antibody, sirtuin type 3 antibody, "silent mating type information regulation 2, S.cerevisiae, homolog 3 antibody", SIR2-like protein 3 antibody, "NAD-dependent deacetylase sirtuin-3, mitochondrial antibody", sirtuin 3 antibody

Background

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX106292_IFA_19011506_996.jpg

GTX106292 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using SIRT3(GTX106292) antibody at 1:200 dilution.

GTX106292_39701_IHC_19011506_323.jpg

GTX106292 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SIRT3(GTX106292) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX106292_WB_19011506_945.jpg

GTX106292 WB Image

Sample(30 µg of whole cell lysate)
A:Hep G2(GTX27900)
10% SDS PAGE
GTX106292 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106292_42172_20151013_IHC-P_19011506_670.jpg

GTX106292 IHC-P Image

SIRT3 antibody [C3], C-term detects SIRT3 protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
SIRT3 antibody [C3], C-term (GTX106292) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf