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SIRT3 antibody

KO/KD
Anti-SIRT3 antibody used in Western Blot (WB). GTX33499
Anti-SIRT3 antibody used in Western Blot (WB). GTX33499
Anti-SIRT3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33499
Anti-SIRT3 antibody used in Western Blot (WB). GTX33499
Anti-SIRT3 antibody used in IHC (Paraffin sections) (IHC-P). GTX33499
Anti-SIRT3 antibody used in IHC (Paraffin sections) (IHC-P). GTX33499
Anti-SIRT3 antibody used in IHC (Paraffin sections) (IHC-P). GTX33499

Cat. No. GTX33499

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all SIRT3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:50 - 1:100
IHC-P 1:20 - 1:50
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human SIRT3 (NP_036371.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887716

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sirtuin 3 , SIR2L3

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SIRT3 antibody used in Western Blot (WB). GTX33499

GTX33499 WB Image

KO/KD

WB analysis of 293T cell lysate using GTX33499 SIRT3 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-SIRT3 antibody used in Western Blot (WB). GTX33499

GTX33499 WB Image

WB analysis of various sample lysates using GTX33499 SIRT3 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-SIRT3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33499

GTX33499 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX33499 SIRT3 antibody.
Blue : DAPI
Dilution : 1:100

Anti-SIRT3 antibody used in Western Blot (WB). GTX33499

GTX33499 WB Image

WB analysis of various sample lysates using GTX33499 SIRT3 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-SIRT3 antibody used in IHC (Paraffin sections) (IHC-P). GTX33499

GTX33499 IHC-P Image

IHC-P analysis of human thyroid cancer tissue using GTX33499 SIRT3 antibody.
Dilution : 1:100

Anti-SIRT3 antibody used in IHC (Paraffin sections) (IHC-P). GTX33499

GTX33499 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX33499 SIRT3 antibody.
Dilution : 1:100

Anti-SIRT3 antibody used in IHC (Paraffin sections) (IHC-P). GTX33499

GTX33499 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX33499 SIRT3 antibody.
Dilution : 1:100

REFERENCE

Application Reference

Ruiz Ram穩rez A et al. Am J Physiol Renal Physiol 2019; (Epub) Kidney dysfunction induced by sucrose-rich diet in rat involves mitochondria ROS generation, cardiolipin changes and the decline of autophagy protein markers.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399