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SIRT4 antibody

Anti-SIRT4 antibody used in Western Blot (WB). GTX10140
Anti-SIRT4 antibody used in IHC (Paraffin sections) (IHC-P). GTX10140

Cat No. GTX10140

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
50 μg ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
IHC-P 2-3μg/ml

Note :

IHC-P
Skeletal Muscle shows spotty staining of striated fibres in longitudinal section.

Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX10140-PEP).

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence SRCGELLPLIDPC, from the C Terminus of the protein sequence according to NP_036372.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Sirtuin 4,Sir2L4,Sirt4

Background

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-SIRT4 antibody used in Western Blot (WB). GTX10140

GTX10140 WB Image

WB analysis of human kidney lysate using GTX10140 SIRT4 antibody.
Dilution : 1µg/ml
Loading : 35µg protein in RIPA buffer

Anti-SIRT4 antibody used in IHC (Paraffin sections) (IHC-P). GTX10140

GTX10140 IHC-P Image

IHC-P analysis of human skeletal muscle using GTX10140 SIRT4 antibody.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5µg/ml

REFERENCE
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REVIEW
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Package List Price ($)
$ 319