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SKA1 antibody

Anti-SKA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX119803
Anti-SKA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX119803
Anti-SKA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX119803

Cat. No. GTX119803

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SKA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SKA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617818

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

spindle and kinetochore associated complex subunit 1 , C18orf24

Cellular Localization

Spindle , Kinetochore

Background

Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the interaction with microtubules.

Database

Research Area

DATA IMAGES

Anti-SKA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX119803

GTX119803 IHC-P Image

C18orf24 antibody detects C18orf24 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
C18orf24 antibody (GTX119803) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SKA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX119803

GTX119803 IHC-P Image

C18orf24 antibody detects C18orf24 protein at cytoplasm in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
C18orf24 antibody (GTX119803) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SKA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX119803

GTX119803 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using C18orf24(GTX119803) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Maciejowski J et al. Dev Cell 2017; 41 (2) : 143-156.e6 Mps1 Regulates Kinetochore-Microtubule Attachment Stability via the Ska Complex to Ensure Error-Free Chromosome Segregation.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169