HCT116 whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SKI antibody (GTX133764) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

HCT116 whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with SKI antibody (GTX133764) diluted at 1:1000 and competitor's antibody (sc-9140) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.