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SKI antibody

Anti-SKI antibody used in IHC (Paraffin sections) (IHC-P). GTX85522
Anti-SKI antibody used in Western Blot (WB). GTX85522

Cat. No. GTX85522

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all SKI products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 20 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

Human Kidney Tissue Slide , HeLa Cell Lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Ski antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human Ski.The immunogen is located within the first 50 amino acids of Ski.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SKI proto-oncogene , SGS , SKV

Cellular Localization

Nucleus

Background

TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Ski functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. Ski will also bind to the Smad proteins specific to bone morphogenic proteins (BMPs) and block BMP signaling in mammalian cells.

Database

Research Area

DATA IMAGES

Anti-SKI antibody used in IHC (Paraffin sections) (IHC-P). GTX85522

GTX85522 IHC-P Image

IHC-P analysis of human kidney tissue using GTX85522 SKI antibody.
Working concentration : 20 μg/ml

Anti-SKI antibody used in Western Blot (WB). GTX85522

GTX85522 WB Image

WB analysis of HeLa cell lysate using GTX85522 SKI antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399