GeneTex
United States (US)

SKI2W antibody

Anti-SKI2W antibody used in Western Blot (WB). GTX31599
Anti-SKI2W antibody used in IHC (Paraffin sections) (IHC-P). GTX31599
Anti-SKI2W antibody used in IHC (Paraffin sections) (IHC-P). GTX31599

Cat No. GTX31599

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

138 kDa. ( Note )

Positive Control

Human Stomach Tissue Slide(GTX21753) , Mouse Stomach Tissue Lysate

Specificity/Sensitivity

At least two isoforms of SKI2W are known to exist; this antibody will detect both isoforms.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

SKI2W antibody was raised against a 16 amino acid peptide near the amino terminus of human SKI2W.The immunogen is located within amino acids 60 - 110 of SKI2W.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ski2 Like Rna Helicase,170A,Ddx13,Hlp,Ski2,Ski2W,Skiv2,Skiv2L1,Thes2,Skiv2L

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a human homologue of yeast SKI2 and may be involved in antiviral activity by blocking translation of poly(A) deficient mRNAs. This gene is located in the class III region of the major histocompatibility complex. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-SKI2W antibody used in Western Blot (WB). GTX31599

GTX31599 WB Image

WB analysis of mouse stomach tissue lysate using GTX31599 SKI2W antibody.
Working concentration : 1 μg/ml

Anti-SKI2W antibody used in IHC (Paraffin sections) (IHC-P). GTX31599

GTX31599 IHC-P Image

IHC-P analysis of human stomach tissue using GTX31599 SKI2W antibody.
Working concentration : 5 μg/ml

Anti-SKI2W antibody used in IHC (Paraffin sections) (IHC-P). GTX31599

GTX31599 IHC-P Image

IHC-P analysis of human stomach tissue using GTX31599 SKI2W antibody.
Working concentration : 20 μg/ml

Package List Price ($)
$ 329