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SKIP antibody

Anti-SKIP antibody used in IHC (Paraffin sections) (IHC-P). GTX32681
Anti-SKIP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32681
Anti-SKIP antibody used in IHC (Paraffin sections) (IHC-P). GTX32681
Anti-SKIP antibody used in Western Blot (WB). GTX32681
Anti-SKIP antibody used in IHC (Paraffin sections) (IHC-P). GTX32681

Cat. No. GTX32681

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all SKIP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 149-448 of human INPP5K (NP_057616.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887674

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inositol polyphosphate-5-phosphatase K , MDCCAID , PPS , SKIP

Cellular Localization

Endoplasmic reticulum

Background

This gene encodes a protein with 5-phosphatase activity toward polyphosphate inositol. The protein localizes to the cytosol in regions lacking actin stress fibers. It is thought that this protein may negatively regulate the actin cytoskeleton. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-SKIP antibody used in IHC (Paraffin sections) (IHC-P). GTX32681

GTX32681 IHC-P Image

IHC-P analysis of rat brain tissue using GTX32681 SKIP antibody.
Dilution : 1:100

Anti-SKIP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32681

GTX32681 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX32681 SKIP antibody.
Blue : DAPI
Dilution : 1:100

Anti-SKIP antibody used in IHC (Paraffin sections) (IHC-P). GTX32681

GTX32681 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX32681 SKIP antibody.
Dilution : 1:100

Anti-SKIP antibody used in Western Blot (WB). GTX32681

GTX32681 WB Image

WB analysis of various sample lysates using GTX32681 SKIP antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-SKIP antibody used in IHC (Paraffin sections) (IHC-P). GTX32681

GTX32681 IHC-P Image

IHC-P analysis of human stomach tissue using GTX32681 SKIP antibody.
Dilution : 1:100

REFERENCE

Application Reference

Dong R et al. J Cell Biol 2018; (Epub) The inositol 5-phosphatase INPP5K participates in the fine control of ER organization.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399