GeneTex
United States (US)

SKP1 Antibody

p19-Skp1-antibody__GTX10546-1_18121411_160.jpg

Cat No. GTX10546

Host Rabbit
Clonality Polyclonal
Application WB, ELISA, IHC
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ELISA 1:2000 - 1:10000
IHC Assay dependent
Not tested in other applications.

Calculated MW

19 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

85mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 152-163 of Human SKP1 (C-terminus) coupled to KLH.

Purification

Antiserum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

OCP2 Antibody , TCEB1L Antibody , P19A Antibody , OCP II Antibody , SKP1A Antibody , EMC19 Antibody

Background

Skp1 is an F-box protein which functions as a substrate recognition component of the SCF ubiquitin ligase complex. It binds to cyclin F, S-phase kinase-associated protein 2, and other regulatory proteins involved in ubiquitin proteolysis through an F-box motif. It is also involved in the control of beta-catenin levels the activity of beta-catenin dependent TCF transcription factors. Studies have also characterized the protein as an RNA polymerase II elongation factor.

Database

Research Area

DATA IMAGES
p19-Skp1-antibody__GTX10546-1_18121411_160.jpg

GTX10546 Image

Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch.

Package List Price ($)
$ 319