APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/1000 |
| 1/200 - 1/1000 |
| 1/200 - 1/400 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.03% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified recombinant fragment of human SKP1 expressed in E. Coli.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
S-phase kinase associated protein 1 , EMC19 , OCP-II , OCP2 , SKP1A , TCEB1L , p19A
Background
This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-SKP1 antibody [4E11] used in IHC (Paraffin sections) (IHC-P). GTX60525](/templates/default/js/lightbox/images/loading.gif "Anti-SKP1 antibody [4E11] used in IHC (Paraffin sections) (IHC-P). GTX60525")
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GTX60525 IHC-P Image
IHC-P analysis of rectum cancer tissue using GTX60525 SKP1 antibody [4E11].
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GTX60525 FACS Image
FACS analysis of NIH3T3 cells using GTX60525 SKP1 antibody [4E11].
Green : SKP1
Red : negative control
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GTX60525 ELISA Image
ELISA analysis of antigen using GTX60525 SKP1 antibody [4E11].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
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GTX60525 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX60525 SKP1 antibody [4E11].
Green : SKP1
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments
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GTX60525 WB Image
WB analysis of human SKP1 (AA: 1-160) recombinant protein using GTX60525 SKP1 antibody [4E11].
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GTX60525 WB Image
WB analysis of HeLa (1), NIH3T3 (2), A431 (3), RAJI (4), PC-12 (5), Cos7 (6), MCF-7 (7) and HepG2 (8) cell lysate using GTX60525 SKP1 antibody [4E11].
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REFERENCE
There are currently no references for SKP1 antibody [4E11] (GTX60525). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SKP1 antibody [4E11] (GTX60525). Be the first to share your experience with this product.
![Anti-SKP1 antibody [4E11] used in IHC (Paraffin sections) (IHC-P). GTX60525](/upload/website/prouct_img/small/GTX60525/GTX60525_20170912_IHC-P_w_23061123_788.webp "Anti-SKP1 antibody [4E11] used in IHC (Paraffin sections) (IHC-P). GTX60525")
![Anti-SKP1 antibody [4E11] used in Flow cytometry (FACS). GTX60525](/upload/website/prouct_img/small/GTX60525/GTX60525_20170912_FACS_w_23061123_836.webp "Anti-SKP1 antibody [4E11] used in Flow cytometry (FACS). GTX60525")
![Anti-SKP1 antibody [4E11] used in ELISA (ELISA). GTX60525](/upload/website/prouct_img/small/GTX60525/GTX60525_20170912_ELISA_w_23061123_271.webp "Anti-SKP1 antibody [4E11] used in ELISA (ELISA). GTX60525")
![Anti-SKP1 antibody [4E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60525](/upload/website/prouct_img/small/GTX60525/GTX60525_20170912_ICCIF_w_23061123_700.webp "Anti-SKP1 antibody [4E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60525")
![Anti-SKP1 antibody [4E11] used in Western Blot (WB). GTX60525](/upload/website/prouct_img/small/GTX60525/GTX60525_20170912_WB_1_w_23061123_541.webp "Anti-SKP1 antibody [4E11] used in Western Blot (WB). GTX60525")
![Anti-SKP1 antibody [4E11] used in Western Blot (WB). GTX60525](/upload/website/prouct_img/small/GTX60525/GTX60525_20170912_WB_w_23061123_130.webp "Anti-SKP1 antibody [4E11] used in Western Blot (WB). GTX60525")
![Anti-SKP1 antibody [4E11] used in IHC (Paraffin sections) (IHC-P). GTX60525](/upload/website/prouct_img/normal/GTX60525/GTX60525_20170912_IHC-P_w_23061123_788.webp "Anti-SKP1 antibody [4E11] used in IHC (Paraffin sections) (IHC-P). GTX60525")
![Anti-SKP1 antibody [4E11] used in Flow cytometry (FACS). GTX60525](/upload/website/prouct_img/normal/GTX60525/GTX60525_20170912_FACS_w_23061123_836.webp "Anti-SKP1 antibody [4E11] used in Flow cytometry (FACS). GTX60525")
![Anti-SKP1 antibody [4E11] used in ELISA (ELISA). GTX60525](/upload/website/prouct_img/normal/GTX60525/GTX60525_20170912_ELISA_w_23061123_271.webp "Anti-SKP1 antibody [4E11] used in ELISA (ELISA). GTX60525")
![Anti-SKP1 antibody [4E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60525](/upload/website/prouct_img/normal/GTX60525/GTX60525_20170912_ICCIF_w_23061123_700.webp "Anti-SKP1 antibody [4E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60525")
![Anti-SKP1 antibody [4E11] used in Western Blot (WB). GTX60525](/upload/website/prouct_img/normal/GTX60525/GTX60525_20170912_WB_1_w_23061123_541.webp "Anti-SKP1 antibody [4E11] used in Western Blot (WB). GTX60525")
![Anti-SKP1 antibody [4E11] used in Western Blot (WB). GTX60525](/upload/website/prouct_img/normal/GTX60525/GTX60525_20170912_WB_w_23061123_130.webp "Anti-SKP1 antibody [4E11] used in Western Blot (WB). GTX60525")