APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
NIH-3T3 , A549 , H1299 , HCT116 , MCF-7
Predict Reactivity
Rat, Zebrafish, Sheep, Bovine, Dog, Chicken, Guinea pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human SKP1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11165159
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
S-phase kinase associated protein 1 , EMC19 , OCP-II , OCP2 , SKP1A , TCEB1L , p19A
Background
This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX106675 IHC-P Image
SKP1 antibody detects SKP1 protein at cytosol and nucleus on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
SKP1 antibody (GTX106675) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX106675 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SKP1 antibody (GTX106675) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX106675 WB Image
Sample (30 μg of whole cell lysate)
A: NIH-3T3
12% SDS PAGE
GTX106675 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX106675 WB Image
Sample (30 μg of whole cell lysate)
A: A549
B: H1299
C: HCT116
D: MCF-7
12% SDS PAGE
GTX106675 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
REVIEW
SKP1 antibody
Cat. No. GTX106675
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Rating
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( Average 5 based on 2 users reviews)
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Application
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Western Blot(WB)
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( Average 5 based on 2 users reviews)
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Date :
Anonymous submitted on 01-Aug-2023
Application Tested :
WB
Sample Species :
Ms
Sample :
RAW264.7 cells with/without LPS treatment
Amount used :
5-10μg
Blocking :
Blocking one (nakalai tesque), 20°C, 1Hr
Primary Antibody :
1:1000, 20°C, 1Hr
Date :
Anonymous submitted on 01-Oct-2018
Application Tested :
WB
Sample Species :
Hu
Sample :
Panc-1 cells
Amount used :
30 μg
Blocking :
5% milk, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Lane Description :
Lane 1: GFP stable cell line, Lane 2: P6 stable cell line
