GeneTex
United States (US)

SKP1 antibody

GTX106675_40702_WB_M_19011506_625.jpg
GTX106675_40702_WB_19011506_762.jpg
GTX106675_40702_IHC_M_19011506_982.jpg

Cat No. GTX106675

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , MCF-7 , NIH-3T3

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Dog, Chicken, Guinea pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SKP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

EMC19 antibody, MGC34403 antibody, OCP-II antibody, OCP2 antibody, SKP1A antibody, TCEB1L antibody, p19A antibody, SKP1 antibody, p19skp1 antibody, cyclin-A/CDK2-associated protein p19 antibody, cyclin A/CDK2-associated protein p19 antibody, SIII antibody, OCP-2 antibody, S-phase kinase-associated protein 1 antibody, RNA polymerase II elongation factor-like protein OCP2 antibody, organ of Corti protein 2 antibody, cyclin A/CDK2-associated p19 antibody, transcription elongation factor B antibody, organ of Corti protein II antibody

Background

This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106675_40702_WB_M_19011506_625.jpg

GTX106675 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
12% SDS PAGE
GTX106675 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106675_40702_WB_19011506_762.jpg

GTX106675 WB Image

Sample (30 µg of whole cell lysate)
A: A549
B: H1299
C: HCT116
D: MCF-7
12% SDS PAGE
GTX106675 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106675_40702_IHC_M_19011506_982.jpg

GTX106675 IHC-P Image

SKP1 antibody detects SKP1 protein at cytosol and nucleus on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
SKP1 antibody (GTX106675) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REVIEW

SKP1 antibody Cat No. GTX106675

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 01-Oct-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : Panc-1 cells
Amount used : 30 μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Lane Description : Lane 1: GFP stable cell line, Lane 2: P6 stable cell line
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169