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SKP2 antibody

Anti-SKP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64578
Anti-SKP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64578
Anti-SKP2 antibody used in Western Blot (WB). GTX64578

Cat. No. GTX64578

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all SKP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide of human SKP2

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

S-phase kinase associated protein 2 , FBL1 , FBXL1 , FLB1 , p45

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class; in addition to an F-box, this protein contains 10 tandem leucine-rich repeats. This protein is an essential element of the cyclin A-CDK2 S-phase kinase. It specifically recognizes phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also referred to as p27 or KIP1) predominantly in S phase and interacts with S-phase kinase-associated protein 1 (SKP1 or p19). In addition, this gene is established as a protooncogene causally involved in the pathogenesis of lymphomas. Alternative splicing of this gene generates three transcript variants encoding different isoforms. [provided by RefSeq, Jul 2011]

Database

Research Area

DATA IMAGES

Anti-SKP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64578

GTX64578 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX64578 SKP2 antibody.
Blue : DAPI
Dilution : 1:100

Anti-SKP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64578

GTX64578 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX64578 SKP2 antibody.
Dilution : 1:100

Anti-SKP2 antibody used in Western Blot (WB). GTX64578

GTX64578 WB Image

WB analysis of various sample lysates using GTX64578 SKP2 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399