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SLBP antibody

Anti-SLBP antibody used in Western Blot (WB). GTX130597
Anti-SLBP antibody used in Western Blot (WB). GTX130597
Anti-SLBP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130597

Cat No. GTX130597

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

293T , HepG2 , HepG2 cytoplasm and membrane fraction extract , SLBP shRNA-transfected 293T

Predict Reactivity

Mouse, Rat, Bovine, Pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.86 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SLBP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

stem-loop binding protein,HBP

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a protein that binds to the stem-loop structure in replication-dependent histone mRNAs. Histone mRNAs do not contain introns or polyadenylation signals, and are processed by endonucleolytic cleavage. The stem-loop structure is essential for efficient processing but this structure also controls the transport, translation and stability of histone mRNAs. Expression of the protein is regulated during the cell cycle, increasing more than 10-fold during the latter part of G1. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-SLBP antibody used in Western Blot (WB). GTX130597

GTX130597 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SLBP antibody (GTX130597) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SLBP antibody used in Western Blot (WB). GTX130597

GTX130597 WB Image

HepG2 whole cell and cytoplasm extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SLBP antibody (GTX130597) diluted at 1:1000.

Anti-SLBP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130597

GTX130597 ICC/IF Image

SLBP antibody detects SLBP protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: Hep G2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SLBP protein stained by SLBP antibody (GTX130597) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169