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SLC12A6 antibody, Internal

Anti-SLC12A6 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88947
Anti-SLC12A6 antibody, Internal used in Western Blot (WB). GTX88947

Cat. No. GTX88947

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($419)
See all SLC12A6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.3μg/ml
IHC-P 2-4μg/ml

Note :

IHC-P
Human Heart shows staining of myocardial fibrils in transverse section.
Not tested in other applications.

Calculated MW

128 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88947-PEP).

Product Note

This antibody is expected to recognise all reported isofoms (NP_598408.1; NP_005126.1; NP_001035959.1; NP_001035961.1; NP_001035962.1)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence SRHIDVCSKTKE, from the internal region of the protein sequence according to NP_001035959.1; NP_001035960.1; NP_001035961.1; NP_001035962.1; NP_005126.1; NP_598408.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 12 member 6 , ACCPN , KCC3 , KCC3A , KCC3B

Background

This gene is a member of the K-Cl cotransporter (KCC) family. K-Cl cotransporters are integral membrane proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The proteins encoded by this gene are activated by cell swelling induced by hypotonic conditions. Alternate splicing results in multiple transcript variants encoding different isoforms. Mutations in this gene are associated with agenesis of the corpus callosum with peripheral neuropathy. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SLC12A6 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88947

GTX88947 IHC-P Image

IHC-P analysis of human heart using GTX88947 SLC12A6 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml

Anti-SLC12A6 antibody, Internal used in Western Blot (WB). GTX88947

GTX88947 WB Image

WB analysis of human heart lysate using GTX88947 SLC12A6 antibody, Internal.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 419