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SLC20A1 antibody [N3C2], Internal

Orthogonal
Anti-SLC20A1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105062
Anti-SLC20A1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105062
Anti-SLC20A1 antibody [N3C2], Internal used in Western Blot (WB). GTX105062

Cat. No. GTX105062

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SLC20A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Cat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SLC20A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951921

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 20 member 1 , GLVR1 , Glvr-1 , PIT1 , PiT-1

Cellular Localization

Membrane; Multi-pass membrane protein

Background

Retrovirus receptors allow infection of human and murine cells by various retroviruses. The receptors that have been identified at the molecular level include CD4 (MIM 186940) for human immunodeficiency virus, Rec1 for murine ecotropic virus, and GLVR1 for gibbon ape leukemia virus (see MIM 182090). These 3 proteins show no homology to one another at the DNA or protein level. GLVR1 is a sodium-dependent phosphate symporter.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-SLC20A1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105062

GTX105062 IHC-P Image

SLC20A1 antibody [N3C2], Internal detects SLC20A1 protein at membrane and cytoplasm on mouse mammary gland by immunohistochemical analysis.
Sample: Paraffin-embedded mouse mammary gland.
SLC20A1 antibody [N3C2], Internal (GTX105062) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SLC20A1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105062

GTX105062 IHC-P Image

SLC20A1 antibody [N3C2], Internal detects SLC20A1 protein at cytosol and membrane on AGS xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded AGS xenograft.
SLC20A1 antibody [N3C2], Internal (GTX105062) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SLC20A1 antibody [N3C2], Internal used in Western Blot (WB). GTX105062

GTX105062 WB Image

Orthogonal

Unboiled U87-MG cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SLC20A1 antibody [N3C2], Internal (GTX105062) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

REFERENCE

Application Reference

Hasegawa T et al. Endocrinology 2023; 164 (4) Evocalcet Rescues Secondary Hyperparathyroidism-driven Cortical Porosity in CKD Male Rats.
Application : ICC/IF
Reactivity : Mouse
Nonoyama S et al. Cells 2021; 10 (11) Development and Characterization of Alkaline Phosphatase-Positive Human Umbilical Cord Perivascular Cells.
Application : WB
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169