GeneTex
United States (US)

SLC25A1 antibody [N1C1]

GTX102191_40898_20170608_WB_19011506_956.jpg
GTX102191_42354_20170524_IFA_19011506_637.jpg
GTX102191_40898_20170608_WB_R_liver_19011506_616.jpg
GTX102191_40898_20170608_WB_M_cerebellum_19011506_380.jpg

Cat No. GTX102191

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

Huh-7 , HepG2 , mouse cerebellum , rat liver

Predict Reactivity

Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SLC25A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CTP antibody, SEA antibody, SLC20A3 antibody, SLC25A1 antibody, citrate transport protein antibody, "solute carrier family 20 (mitochondrial citrate transporter), member 3 antibody", "tricarboxylate transport protein, mitochondrial antibody", tricarboxylate carrier protein antibody, "solute carrier family 25 (mitochondrial carrier, citrate transporter), member 1 antibody"

Cellular Localization

Mitochondrion inner membrane; Multi-pass membrane protein

Background

The mitochondrial tricarboxylate transporter (also called citrate transport protein, or CTP) is responsible for the movement of citrate across the mitochondrial inner membrane (Kaplan et al., 1993 [PubMed 8514800]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX102191_40898_20170608_WB_19011506_956.jpg

GTX102191 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SLC25A1 antibody [N1C1] (GTX102191) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102191_42354_20170524_IFA_19011506_637.jpg

GTX102191 ICC/IF Image

SLC25A1 antibody [N1C1] detects SLC25A1 protein at mitochondria by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: SLC25A1 protein stained by SLC25A1 antibody [N1C1] (GTX102191) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX102191_40898_20170608_WB_R_liver_19011506_616.jpg

GTX102191 WB Image

Rat tissue extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with SLC25A1 antibody [N1C1] (GTX102191) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102191_40898_20170608_WB_M_cerebellum_19011506_380.jpg

GTX102191 WB Image

Mouse tissue extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with SLC25A1 antibody [N1C1] (GTX102191) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169