APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:10000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat , Raji , rat kidney
Predict Reactivity
Mouse, Zebrafish, Sheep, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human SLC25A11. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11174834
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
solute carrier family 25 member 11 , OGC , SLC20A4
Cellular Localization
Mitochondrion inner membrane; Multi-pass membrane protein
Background
The oxoglutarate/malate carrier transports 2-oxoglutarate across the inner membranes of mitochondria in an electroneutral exchange for malate or other dicarboxylic acids (summary by Iacobazzi et al., 1992 [PubMed 1457818]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX101761 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SLC25A11 antibody (GTX101761) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101761 WB Image
Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SLC25A11 antibody (GTX101761) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101761 IHC-P Image
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using SLC25A11(GTX101761) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
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REVIEW
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