APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse liver , rat liver , 293T , A431 , HeLa , HepG2
Predict Reactivity
Chicken, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.61 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human SLC25A13. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951925
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
solute carrier family 25 member 13 , ARALAR2 , CITRIN , CTLN2
Cellular Localization
Mitochondrion inner membrane
Background
This gene is a member of the mitochondrial carrier family. The encoded protein contains four EF-hand Ca(2+) binding motifs in the N-terminal domain, and localizes to mitochondria. The protein catalyzes the exchange of aspartate for glutamate and a proton across the inner mitochondrial membrane, and is stimulated by calcium on the external side of the inner mitochondrial membrane. Mutations in this gene result in citrullinemia, type II. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX109001 IHC-P Image
SLC25A13 antibody [N3C2], Internal detects SLC25A13 protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. SLC25A13 antibody [N3C2], Internal (GTX109001) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX109001 WB Image
Wild-type (WT) and SLC25A13 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SLC25A13 antibody [N3C2], Internal (GTX109001) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX109001 WB Image
Sample (50 μg of whole cell lysate) A: mouse liver 7.5% SDS PAGE GTX109001 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX109001 ICC/IF Image
SLC25A13 antibody [N3C2], Internal detects SLC25A13 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SLC25A13 protein stained by SLC25A13 antibody [N3C2], Internal (GTX109001) diluted at 1:500. Blue: Hoechst 33342 staining.
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GTX109001 IHC-P Image
SLC25A13 antibody [N3C2], Internal detects SLC25A13 protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. SLC25A13 antibody [N3C2], Internal (GTX109001) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX109001 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SLC25A13 antibody [N3C2], Internal (GTX109001) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX109001 WB Image
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SLC25A13 antibody [N3C2], Internal (GTX109001) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX109001 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with SLC25A13 antibody [N3C2], Internal (GTX109001) diluted at 1:1000 and competitor's antibody (10789-1-AP) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX109001 IHC-P Image
Immunohistochemical analysis of paraffin-embedded N87 xenograft, using SLC25A13(GTX109001) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
There are currently no references for SLC25A13 antibody [N3C2], Internal (GTX109001). Be the first to share your publications with this product.
REVIEW
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