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SLC25A6 antibody

Anti-SLC25A6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110983
Anti-SLC25A6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110983
Anti-SLC25A6 antibody used in Western Blot (WB). GTX110983
Anti-SLC25A6 antibody used in Immunoprecipitation (IP). GTX110983
Anti-SLC25A6 antibody used in IHC (Paraffin sections) (IHC-P). GTX110983
Anti-SLC25A6 antibody used in IHC (Paraffin sections) (IHC-P). GTX110983
Anti-SLC25A6 antibody used in IHC (Paraffin sections) (IHC-P). GTX110983
Anti-SLC25A6 antibody used in IHC (Paraffin sections) (IHC-P). GTX110983
Anti-SLC25A6 antibody used in IHC (Paraffin sections) (IHC-P). GTX110983

Cat. No. GTX110983

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Predict Reactivity

Zebrafish, Sheep, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SLC25A6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169465

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 25 member 6 , AAC3 , ANT , ANT 2 , ANT 3 , ANT3 , ANT3Y

Cellular Localization

Mitochondrion inner membrane

Background

This gene is a member of the mitochondrial carrier subfamily of solute carrier protein genes. The product of this gene functions as a gated pore that translocates ADP from the mitochondrial matrix into the cytoplasm. The protein is implicated in the function of the permability transition pore complex (PTPC), which regulates the release of mitochondrial products that induce apoptosis. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq]

Database

  • Gene ID: 293 SLC25A6
  • UniProt: P12236 SLC25A6

Research Area

DATA IMAGES

Anti-SLC25A6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110983

GTX110983 ICC/IF Image

SLC25A6 antibody detects SLC25A6 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: SLC25A6 stained by SLC25A6 antibody (GTX110983) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-SLC25A6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110983

GTX110983 ICC/IF Image

SLC25A6 antibody detects SLC25A6 protein at mitochondria by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SLC25A6 protein stained by SLC25A6 antibody (GTX110983) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-SLC25A6 antibody used in Western Blot (WB). GTX110983

GTX110983 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SLC25A6 antibody (GTX110983) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SLC25A6 antibody used in Immunoprecipitation (IP). GTX110983

GTX110983 IP Image

Immunoprecipitation of SLC25A6 protein from 293T whole cell extracts using 5 μg of SLC25A6 antibody (GTX110983).
Western blot analysis was performed using SLC25A6 antibody (GTX110983).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-SLC25A6 antibody used in IHC (Paraffin sections) (IHC-P). GTX110983

GTX110983 IHC-P Image

SLC25A6 antibody detects SLC25A6 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat muscle.
SLC25A6 stained by SLC25A6 antibody (GTX110983) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SLC25A6 antibody used in IHC (Paraffin sections) (IHC-P). GTX110983

GTX110983 IHC-P Image

SLC25A6 antibody detects SLC25A6 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
SLC25A6 stained by SLC25A6 antibody (GTX110983) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SLC25A6 antibody used in IHC (Paraffin sections) (IHC-P). GTX110983

GTX110983 IHC-P Image

SLC25A6 antibody detects SLC25A6 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
SLC25A6 stained by SLC25A6 antibody (GTX110983) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SLC25A6 antibody used in IHC (Paraffin sections) (IHC-P). GTX110983

GTX110983 IHC-P Image

SLC25A6 antibody detects SLC25A6 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
SLC25A6 stained by SLC25A6 antibody (GTX110983) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SLC25A6 antibody used in IHC (Paraffin sections) (IHC-P). GTX110983

GTX110983 IHC-P Image

SLC25A6 antibody detects SLC25A6 protein at mitochondria in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
SLC25A6 antibody (GTX110983) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169