GeneTex
United States (US)

SLC27A2 antibody [C1C3]

GTX115526_40415_20180810_WB_R_liver_18121410_910.jpg
GTX115526_40415_WB_18121410_905.jpg
GTX115526_40415_WB_M_liver_18121410_437.jpg
GTX115526_40415_IHC_18121410_753.jpg

Cat No. GTX115526

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

293T , mouse liver , rat liver

Predict Reactivity

Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SLC27A2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Solute Carrier Family 27 Member 2,Acsvl1,Facvl1,Fatp2,Hst17226,Vlacs,Vlcs,Hfacvl1,Slc27A2

Cellular Localization

Endoplasmic reticulum membrane; Multi-pass membrane protein , Peroxisome membrane; Multi-pass membrane protein

Background

The protein encoded by this gene is an isozyme of long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme activates long-chain, branched-chain and very-long-chain fatty acids containing 22 or more carbons to their CoA derivatives. It is expressed primarily in liver and kidney, and is present in both endoplasmic reticulum and peroxisomes but not in mitochondria. Its decreased peroxisomal enzyme activity is in part responsible for the biochemical pathology in X-linked adrenoleukodystrophy. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX115526_40415_20180810_WB_R_liver_18121410_910.jpg

GTX115526 WB Image

Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SLC27A2 antibody [C1C3] (GTX115526) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115526_40415_WB_18121410_905.jpg

GTX115526 WB Image

Sample (30 µg of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX115526 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115526_40415_WB_M_liver_18121410_437.jpg

GTX115526 WB Image

Sample (50 µg of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX115526 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115526_40415_IHC_18121410_753.jpg

GTX115526 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using SLC27A2(GTX115526) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REVIEW

SLC27A2 antibody [C1C3] Cat No. GTX115526

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 27-Nov-2014
Score :
Application Tested : WB
Sample Species : Ms
Sample : liver tissue
Amount used : 30μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169