Unboiled HeLa whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SLC38A1 antibody (GTX131854) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.