SLC7A8 antibody [5A9]

Name: SLC7A8 antibody [5A9]
Brand: GeneTex
SKU: GTX83618
Price: 539.00 USD

Anti-SLC7A8 antibody [5A9] used in Immunoprecipitation (IP). GTX83618

Anti-SLC7A8 antibody [5A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83618

Anti-SLC7A8 antibody [5A9] used in Western Blot (WB). GTX83618

Anti-SLC7A8 antibody [5A9] used in Flow cytometry (FACS). GTX83618

Anti-SLC7A8 antibody [5A9] used in IHC (Paraffin sections) (IHC-P). GTX83618

Anti-SLC7A8 antibody [5A9] used in Western Blot and Immunoprecipitation (WB IP). GTX83618

Cat. No. GTX83618

Reference 2

Host	Mouse
Clonality	Monoclonal
Clone Name	5A9
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS, IP
Reactivity	Human, Monkey
Package	100 μl ($539)

See all SLC7A8 products

View product citations for antibody GTX83618 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:1000
ICC/IF	1:100
IHC-P	1:50
FACS	1:100
IP	2ug/500ul

Not tested in other applications.

Calculated MW

58 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human SLC7A8 expression vector .

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_10728197

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 7 member 8 , LAT2 , LPI-PC1

Cellular Localization

Cytoplasm

Background

Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney.

Database

Gene ID: 23428 SLC7A8
UniProt: Q9UHI5 SLC7A8

Research Area

DATA IMAGES

GTX83618 IP Image

IP analysis of DDDDK tagged SLC7A8 overexpressed HEK293T lysate using GTX83618 SLC7A8 antibody [5A9].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

GTX83618 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with SLC7A8 plasmid using GTX83618 SLC7A8 antibody [5A9].

GTX83618 WB Image

WB analysis of HEK293T cells transfected with SLC7A8 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83618 SLC7A8 antibody [5A9].
Loading : 5 ug per lane

GTX83618 WB Image

WB analysis of various cell lines using GTX83618 SLC7A8 antibody [5A9].
Loading : 35 ug per lane
Dilution : 1:200

GTX83618 FACS Image

FACS analysis of HEK293T cells transfected with either SLC7A8 plasmid(Red) or empty vector control plasmid(Blue) using GTX83618 SLC7A8 antibody [5A9].

GTX83618 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX83618 SLC7A8 antibody [5A9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.