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SLD5 antibody [N1C3]

Anti-SLD5 antibody [N1C3] used in Western Blot (WB). GTX119779
Anti-SLD5 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119779
Anti-SLD5 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119779

Cat. No. GTX119779

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SLD5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Observed MW

34 kDa. The observed M.W. is based on the following publication. PMID: 32012389

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SLD5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617594

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GINS complex subunit 4 , SLD5

Cellular Localization

Cytoplasm , Nucleus

Background

The yeast heterotetrameric GINS complex is made up of Sld5, Psf1 (GINS1; MIM 610608), Psf2 (GINS2; MIM 610609), and Psf3 (GINS3; MIM 610610). The formation of the GINS complex is essential for the initiation of DNA replication in yeast and Xenopus egg extracts (Ueno et al., 2005 [PubMed 16287864]). See GINS1 for additional information about the GINS complex.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-SLD5 antibody [N1C3] used in Western Blot (WB). GTX119779

GTX119779 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SLD5 antibody [N1C3] (GTX119779) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SLD5 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119779

GTX119779 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using GINS4(GTX119779) antibody at 1:200 dilution.

Anti-SLD5 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX119779

GTX119779 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GINS4(GTX119779) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Binfeng Liu et al. Mol Med 2021; 27 (1) : 117 Overexpression of GINS4 is associated with poor prognosis and survival in glioma patients.
Application : IHC-P
Reactivity : Human
Shin G et al. J Biol Chem 2019; RecQL4 tethering on the pre-replicative complex induces unscheduled origin activation and replication stress in human cells.
Application : WB
Reactivity : Human
Yang R et al. Oncogene 2019; (Epub) GIAT4RA functions as a tumor suppressor in non-small cell lung cancer by counteracting Uchl3-mediated deubiquitination of LSH.
Application : WB
Reactivity : Human
Yang R et al. J Exp Clin Cancer Res 2019; 38 (1) : 280 LSH interacts with and stabilizes GINS4 transcript that promotes tumourigenesis in non-small cell lung cancer.
Application : WB
Reactivity : Human
Jia W et al. Neurochem Int 2019; 129 : 104465 Embryonic expression of GINS members in the development of the mammalian nervous system.
Application : IHC-P
Reactivity : Mouse
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169