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SLIT1 antibody

Anti-SLIT1 antibody used in Western Blot (WB). GTX134122
Anti-SLIT1 antibody used in Western Blot (WB). GTX134122
Anti-SLIT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134122

Cat. No. GTX134122

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SLIT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

167 kDa. ( Note )

Positive Control

mouse brain

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of mouse SLIT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887222

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

slit guidance ligand 1 , Slil1 , mKIAA0813

Cellular Localization

Secreted

Database

Research Area

DATA IMAGES

Anti-SLIT1 antibody used in Western Blot (WB). GTX134122

GTX134122 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SLIT1 antibody (GTX134122) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SLIT1 antibody used in Western Blot (WB). GTX134122

GTX134122 WB Image

Mouse tissue extract (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SLIT1 antibody (GTX134122) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SLIT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134122

GTX134122 ICC/IF Image

SLIT1 antibody detects SLIT1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SLIT1 stained by SLIT1 antibody (GTX134122) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169