GeneTex
  • Country / Location Selection

United States (US)

SLIT1 antibody

Anti-SLIT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85822
Anti-SLIT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85822
Anti-SLIT1 antibody used in Western Blot (WB). GTX85822

Cat No. GTX85822

Host Rabbit
Clonality Polyclonal
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
Package
50μg ($369)
APPLICATION

Application Note

WB (1:500 - 1:2000), ELISA (1:10000 - 1:50000),IHC-P (5 μg/ml) Human cross reactivity tested by IHC-P only

Calculated MW

167 kDa. ( Note )

Specificity/Sensitivity

aa 487-504 of mouse SLIT-1 protein.
PROPERTIES

Form

Liquid

Buffer

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

A synthetic peptide corresponding to a region within amino acids 487 and 504 of mouse SLIT-1.

Purification

This antibody was purified using Immunoaffinity Chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Slit Guidance Ligand 1,Slil1,Mkiaa0813,Slit1

Background

Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.

Database

Research Area

DATA IMAGES
Anti-SLIT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85822

GTX85822 IHC-P Image

Anti-SLIT1 antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody GTX85822 concentration 5 ug/ml.

Anti-SLIT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85822

GTX85822 IHC-P Image

Anti-SLIT1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody GTX85822 concentration 5 ug/ml.

Anti-SLIT1 antibody used in Western Blot (WB). GTX85822

GTX85822 WB Image

Western blot of Affinity Purified anti-SLIT-1 antibody shows detection of SLIT-1 in rat (lane 1) and mouse (lane 2) brain lysates. The expected molecular weight for SLIT-1 is 168 kD. Approximately 20 ug of each lysates was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-SLIT-1 antibody. Signal was detected using standard techniques. Note: The smaller strong bands observed in this blot are likely SLIT-1 cleavage products. A number of cleavage products for both Slit1 and Slit2 are reported in the literature resulting from alternate splicing and range from ~40kD -160kD

REFERENCE
REVIEW
There are currently no reviews for SLIT1 antibody (GTX85822).
Be the first to share your experience with this product.
Package List Price ($)
$ 369