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SLIT2 antibody

Anti-SLIT2 antibody used in Western Blot (WB). GTX118220
Anti-SLIT2 antibody used in Western Blot (WB). GTX118220
Anti-SLIT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX118220
Anti-SLIT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX118220
Anti-SLIT2 antibody used in Immunoprecipitation (IP). GTX118220
Anti-SLIT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX118220
Anti-SLIT2 antibody used in Western Blot (WB). GTX118220
Anti-SLIT2 antibody used in Western Blot (WB). GTX118220

Cat No. GTX118220

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

170 kDa. ( Note )

Positive Control

IMR32 , SK-N-AS , mouse brain , rat brain , SLIT2-transfected 293T

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SLIT2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618954

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

slit guidance ligand 2 , SLIL3 , Slit-2

Cellular Localization

Secreted

Background

Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration.

Database

Research Area

DATA IMAGES

Anti-SLIT2 antibody used in Western Blot (WB). GTX118220

GTX118220 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SLIT2 antibody (GTX118220) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SLIT2 antibody used in Western Blot (WB). GTX118220

GTX118220 WB Image

SLIT2 antibody detects SLIT2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SLIT2 antibody (GTX118220) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SLIT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX118220

GTX118220 IHC-P Image

SLIT2 antibody detects SLIT2 protein at cytosol and membrane on gastric carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human gastric carcinoma.
SLIT2 antibody (GTX118220) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SLIT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX118220

GTX118220 IHC-P Image

SLIT2 antibody detects SLIT2 protein at cytoplasm in mouse fetal brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fetal brain.
Green: SLIT2 antibody (GTX118220) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SLIT2 antibody used in Immunoprecipitation (IP). GTX118220

GTX118220 IP Image

SLIT2 antibody immunoprecipitates SLIT2 protein in IP experiments. IP Sample: Raji whole cell lysate/extract A : 30 μg whole cell lysate/extract of SLIT2 protein expressing Raji cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of SLIT2 by 3 μg of SLIT2 antibody (GTX118220) 5% SDS-PAGE The immunoprecipitated SLIT2 protein was detected by SLIT2 antibody (GTX118220) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-SLIT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX118220

GTX118220 IHC-P Image

SLIT2 antibody detects SLIT2 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
SLIT2 antibody (GTX118220) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SLIT2 antibody used in Western Blot (WB). GTX118220

GTX118220 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SLIT2 antibody (GTX118220) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SLIT2 antibody used in Western Blot (WB). GTX118220

GTX118220 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SLIT2 antibody (GTX118220) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169