GeneTex
United States (US)

SLK antibody

KO/KD
GTX103840_40884_20180330_WB_KO_watremark_19011506_269.jpg
GTX103840_40884_IHC_19011506_962.jpg
GTX103840_40884_WB_19011506_802.jpg

Cat No. GTX103840

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

143 kDa. ( Note )

Positive Control

293T , A549 , H1299 , HCT116 , MCF-7
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SLK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ste20 Like Kinase,Losk,Stk2,Ba16H23.1,Se20-9,Slk

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES
GTX103840_40884_20180330_WB_KO_watremark_19011506_269.jpg

GTX103840 WB Image

KO/KD

Wild-type (WT) and SLK knockout (KO) 293T cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SLK antibody (GTX103840) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX103840_40884_IHC_19011506_962.jpg

GTX103840 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using SLK(GTX103840) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103840_40884_WB_19011506_802.jpg

GTX103840 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: H1299
C: HCT116
D: MCF-7
5% SDS PAGE
GTX103840 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169